GC Method Development On-site course (Part No: CS-IE-GC-14)

1 day course for up to 10 analysts


- Establishing Method Objectives
- Existing Information - what is known, what should be known
- Chromatographic Theory Overview
- Mechanisms of Separation
- Equilibrium and the Distribution Constant Solute - Stationary Phase Interaction
- Sample Preparation Protocols
- Injection System Choice - spilt / splitless, on-column, direct, PTV, headspace. (Each of the above will be considered with reference to the physical properties of the analyte / matrix / solvent which govern choice)
- Carrier Gas Choice
- Column Choice (stationary phase chemistry choice as governed by the analyte/s)
- Detector System Choice / Operation, including FID, ECD, TCD, MS etc. (Once again, each of the above will be considered with reference to the physical properties of the analyte/matrix/solvent which govern choice)
- Measuring and Optimising Chromatographic Parameters
- Capacity Factor (k), Efficiency (N), Selectivity ( a ) and Resolution (R)
- Temperature Effects
- Appropriate Methods of Quantitation