Brucella Agar 1012 Condalab

Brucella Agar is rich in nutrients and growth factors which makes it suitable to grow and isolate fastidious microorganisms. It can be used to successfully isolate Brucella from diverse specimens contaminated with microflora, both saprophytes and commensals in clinical samples as well as in food. The medium is used to produce clostridial toxins and can be utilised in the isolation of many anaerobes of human and animal origin. Food samples can be inoculated directly on the plates of Brucella Agar, while clinical specimens are more convenient as suspensions or macerations in sterile saline solutions.

Meat peptone and casein peptone provide nitrogen, vitamins, minerals. and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group. Sodium bisulphite is the reducing agent; Sodium chloride supplies essential electrolytes for transport and osmotic balance. Dextrose is the fermentable carbohydrate providing carbon and energy. The addition of blood provides extra growth factors for fastidious microorganisms. Bacteriological agar is the solidifying agent. The addition of the Brucella Supplement (Cat. CL-6017) enhances the medium’s selectivity for the growth of Brucella. For a better growth, Polyenrichment Supplement (Cat. CL-6011) and Polyenrichment CC Supplement (Cat. CL-6071) may be added if required. Brucella species are level 3 pathogens and cause brucellosis, a zoonotic disease. It is usually transmitted through milk, dairy products, meat, and direct contact with infected animals.

Note: To obtain an excellent medium for anaerobes, add 5 mg/ml of hemin and 10 μg/ml of vitamin K1 (fitomenadione) to the basal medium. Inoculate and incubate in anaerobic conditions.