By Emma Foster on Fri 24 April 2020
Learn more about biological buffers and how they help to control pH within tissue culture media.
Sodium bicarbonate is the most commonly used buffer in tissue culture media but there are some disadvantages to using this:
- The pKa of sodium bicarbonate is 6.3 at 37 degrees Celsius which would result in suboptimal buffering throughout the balanced salt solution pH range
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