The breakthrough in recombinant protein production has allowed BioConcept Ltd to produce and supply a new type of Mammalian Cell Culture Media. MAM-PF® known as Mammalian Artificial Media which is a line of media tailor made to satisfy the needs of researchers and producers. This Mammalian Cell Culture Media is Animal Component Free and is in accordance with strict quality guidelines EMA/410/01.
This Cell Culture Media is protein-free, protein hydrolysates free and chemically defined. It can be used for the high cell density cultivation of a variety of cell lines such as CHO (Chinese Hamster Ovary) cells or BHK (Baby Hamster Kidney) cells and high-level expression of recombinant protein. BioConcept holds a certificate for every single component used in the MAM-PF® media series to guarantee an untainted and exceptional final product.
This Mammalian Cell Culture production media is formulated without L-glutamine to avoid problems associated with L-Glutamine degradation including ammonia accumulation. This production media can be obtained with minimal amounts of Phenol Red or without. This serum-free media holds an advantage over serum supplemented culture as it facilitates purification and downstream processing of expressed products. When compared to other commercially available mammalian cell culture production media, MAM-PF® is protein-free and does not contain protein hydrolysates.
|Cat. No.||Product||Size||Serum Free||Protein Free||
|BC-10-02F24-I||MAM-PF ® 2 growth and production medium w/o Phenol Red||500ml||x||x||x||Proprietary|
|BC-10-02F25-I||MAM-PF ® 2 growth and production medium with Phenol Red||500ml||x||x||x||Proprietary|
|BC-10-02F27-I||MAM-PF ® 1 adaptation and growth medium||500ml||x||x||x||Proprietary|
|BC-10-02F33-I||MAM-PF ® 2 Total Selection Medium (w/o L-Gln, L-Trp, L-His, Gly, Hypoxanthine and Thymidine)||500ml||x||x||x||Proprietary|
|BC-10-02P74||MAM-PF ® 7e Powder Media||500ml||x||x||x||Proprietary|
|BC-10-02S80-I||MAM-PF ®77 production medium||Flexible||x||x||x||Proprietary|
|BC-10-02P24-K/L/M/N/W||MAM-PF ® 2 growth and production w/o Phenol Red, w/o L-Gln, w/o NaHCO 3||1L, 5L, 10L, 50L & 100L||x||x||x||Proprietary|
|BC-5-77F01-H||Lipid-Mixture for MAM-PF ® 2 Powder (1000×)||100ml||x||x||x||Proprietary|
CHOSI cells cultured in the Animal Component Free Media, MAM-PF77®, show the fastest growth along with a ~100% and ~40% higher cell density compared to media by Suppliers S and T. When looking at the graph, this corresponds with the final product titers at the end of the fed-batch. All cell cultures in the display were fed with FMS3 in the same feed regime. The higher viability in the stationary phase shows that the glycosylation in Mammalian Cell Culture Production Media, MAMPF77®, was superior.
When mixing the CHO feed mix FMS3 to MAM-PF77®, a cell density of over 100 mio. Cells per litter can be reached. A titer of over 350 mg/L of highly glycosylated follicle stimulating hormone (FSH) is possible to achieve within 14 days of stirring in a bioreactor tank. This demonstrates that the mammalian cell culture production media is a very high-quality product. As determined during the purification process and as shown on the graph, 45% of the product displays an isoform-pattern, low aggregates, and low oxidized forms. Thus meaning, MAM-PF77® can be used to produce quality FSH that fulfils its Ph.Eur. requirements.
The continuous innovation and development of the MAM-PF® mammalian cell culture media has led to an addition called MAM-PF77® cell culture medium and CHO Feed Mixes FMS3 and FMU. MAM-PF® media can increase productivity of Ipilimumab (monoclonal antibody) by up to 5g/L, which is shown in the graph over a 14-day trial. The new expression system is also viable in fed-batch and perfusion systems.
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