FreezerCell™ can be used for a variety of cell types, including stem cells, primary cells, cell lines and yeasts. The FreezerCell™ does not require any additives and only needs to be placed in an ultra deep freezer and the cooling runs at -1°C per minute. Fill the freezing aid with the cryotubes and freeze for approx. 3 hours at -80 °C. Then the samples are ready for transfer to a nitrogen tank. This gentle method ensures a high survival rate of the cells during the freezing process.