Canvax HotBegan™ HS Red-Taq MasterMix P0321

HotBegan™ HS Red-Taq MasterMix, for a specific, highly efficient & reliable amplifications of dna up 160 fg, with visual tracking of dna migration

HotBegan™ HS Red-Taq Master Mix (2x) is an optimized ready-to-use solution containing HotBegan™ Hot Start Taq DNA Polymerase, dNTPs, MgCl2 and Stabilizers. It is inactive at room temperature and only requires addition of template, primers, and water. HotBegan™ Hot Start Taq DNA Polymerase is a Taq DNA Polymerase bound to a proprietary antibody that blocks Polymerase activity until denaturation step occurs. The heat labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and nonspecific products. MasterMix also contains an Agarose Loading Buffer including a Red Dye for visual tracking of DNA migration and a dense compound to facilitate the drop-down of the samples into the well Agarose Gels. Like the Taq polymerase, the enzyme has 5’?3’ polymerase activity and a weak 5’?3’ exonuclease activity but no 3’?5’ exonuclease activity (proofreading). Before enzyme activation none of enzyme activities are detectable.

Main Features:

Accurate & Fast visual Tracking of DNA Migration: thanks to its composition that includes Red Dye Buffer. High specificity: minimizes unspecific amplification. Efficient: prevents or minimizes primer-dimer and nonspecific products. Great sensitivity: amplifies from femptograms of DNA targets. Inactive: at Room Temperature. Optimized Powerful: amplification of targets up to 5 kb. Convenient: available in kits and MasterMix solution. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 500 rxn: - 10 x 1.25 mL HotBegan™ HS Red-Taq MasterMix (2x)* - 1.5 mL 50mM MgCl2 Solution** &nbsp

Applications: PCR fragments amplification for TA or GC Cloning. Design for High Throughput Applications. Amplification from a limited DNA template or low copy number genes.