Horse-Power™ Taq DNA Polymerase, highly purified (>98%) for routine amplifications
Horse-Power™ Taq DNA Polymerase is a pure, versatile and thermostable recombinant enzyme produced in an E. coli strain, which carries the cloned pol gene from Thermus aquaticus. The enzyme has 5’?3’ polymerase activity and a weak 5’?3’ exonuclease activity but no 3’?5’ exonuclease activity (proofreading).
Highest purity: greater than 98% confirmed by SDS-PAGE. Highest quality: high activity, specificity, thermostability and performance in PCR. Highly efficient: reactivation buffer improved. Thermostable: half-life at 94 ºC is 40 minutes. Adds extra nucleotides: preferentially adenine, without template at 3´ends leaving 3´overhangs PCR fragments. Incorporates modified nucleotides: biotinylated, fluorescently labelled, etc. Molecular Weight: 94 kDa. Convenient: available in different concentrations, sizes and solutions. Complete solution: includes MgCl2. Risk-free: product covered by Canvax Quality 100% Guarantee.
Includes: Concentration: 5 U/µL - Includes for 500 U: - 100 µL Horse-Power™ Taq Polymerase (5 U/µL) - 25 mM MgCl2 (1.5 mL) - 1.5 mL Buffer (10x) Concentration: 1 U/µL - Includes for 500 U: - 500 µL Horse-Power™ Taq Polymerase (1 U/µL) - 25 mM MgCl2 (1.5 mL) - 1.5 mL Buffer (10x)
Applications: Routine amplifications. Colony screening (see Horse-Power™ Red-Taq DNA Polymerase). Amplifications up to 5 kb using plasmid, viral or genomic DNA as template. PCR fragments amplification for TA or GC cloning (preferably use a proofreading polymerase for cloning purposes combined with an efficient blunt cloning vector) (see pSpark® DNA Cloning System).