PickMutant™ Site-directed Mutagenesis Kit, for a reliable, robust & highly efficient site-directed mutagenesis based in pcr
PickMutant™ is a reliable, robust and highly efficient PCR-based Mutagenesis Kit. Extremely easy-to-use, the kit allows creating single or multiple point mutations, deletions or insertions using a rapid and easy protocol. All these mutation could be obtained by PCR using a FastPANGEA™ High Fidelity DNA Polymerase and well-designed Mutagenesis primers. The assembled mutagenic PCR fragments is cloned into Canvax™ pSpark® Cloning vector, specially designed to clone blunt PCR fragments with high efficiency or into other vector designing, in this case, an additional specific vector primer pair.
Highly Effective point mutations (single or multiple), deletions or insertions. Easy and fast protocol: it takes less than 3 hours in one step procedure. Cost avoidance: compatible with any bacterial strains or primers. Versatile: compatible with any Cloning vector. Efficient: includes highly efficient pSpark® to clone blunted fragments. Robust: simultaneous assemble and clone of PCR fragments. Risk-free: product covered by Canvax Quality 100% Guarantee.
Includes: Includes for 15 rxn: - 150 µL Proofreading DNA Polymerase MasterMix (2x) - 300 U Glue Enzyme (10 U/µL) - 40 µL Glue Enzyme Buffer (10x) - 5 µL Insert Control DNA - 15 µL pSpark® I (20 ng/µL)
Applications: Site-directed Mutagenesis. Study protein function. Identify enzyme active sites. Design new proteins.