Canvax pOnebyOne™ IV - Lentiviral Bicistronic Mammalian Expression Kit ME0020-S

pOnebyOne™ IV - Lentiviral Bicistronic Mammalian Expression Kit, extremely efficient lineal vector ready-to-clone your pcr gene and express your protein of interest

pOnebyOne™ are extremely efficient, accurate and flexible Bicistronic Mammalian Expression Kits that contains an expression cassette based in 2A sequence Breakthrough technology. The expression cassette of pOnebyOne™ IV - Lentiviral includes the Human elongation factor 1 alpha promoter that precedes 2A sequence in frame with the enhanced green fluorescent protein (eGFP) from Aequoria victoria. eGFP is optimized for brighter and higher expression in mammalian cells, it can be visualized by cytometry or microscopy (Excitation wavelength maximum=488 nm/ Emission wavelength maximum=507 nm). Positive cells can be sorted by means of a sorter cytometer.

Main Features:

Complete solution: a directional cloning vector to clone and produce a protein of interest. Breakthrough technology: based in 2A sequence simultaneous expression of two proteins in mammalian cells (a protein of interest and reporter). Highly efficient: cloning system tested with up to 4 kb inserts. Time-saving cloning process: linearized vector ready-to-mix with your PCR fragment. Easy-to-use: facilitates the selection of positive cells expressing the recombinant gene of interest. Cost avoidance: avoids the use of restriction enzymes. Accurate: proven performance for most common cell lines. Flexible: allows transfection in difficult cell lines. Really low experimental background: lower than 1%. Convenient: available with different resistance marker cassettes. Directional cloning: of PCR with the gene of interest. Reporter checking: stoichiometric amount of your protein of interest and a reporter protein. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL Linearized vector (50 ng/µL) - 40 µL Glue Enzyme (10 u/µL) - 50 µL Glue Enzyme Buffer (10x) - 10 µL Insert Control (30 ng/µL) - 5 µL pOnebyOne™ Control (50 ng/µL)

Applications: Highest generation lentiviral transfer vector to transform replicating and non-replicating cells, including stem and neuronal cells. Vector of choice for short-interfering RNA (siRNA) delivery and for gene therapy.