Canvax G protein-coupled Estrogen Receptor 1 G0549-B

G protein-coupled Estrogen Receptor 1, ExpressMAX™ Human GPCR ORF Clones

G protein-coupled Estrogen Receptor 1 is a member of the GPCR family and is encoded in Humans by the GPER gene. Alternate transcriptional splice variants that encode the same protein have been characterized. It is a member of the rhodopsin-like family and is localized to the endoplasmic reticulum membrane. GPER binds estrogen with high affinity, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus.

Main Features:

Native structure. Wide range: a large collection of ExpressMAX™ GPCR ORF Clones availables. Highest levels of GPCR expression: on surface cell lines, > 50% of TAG detection by cytometry. Wide spectrum: of strong constitutive promoters. Complete solution: it contains all necessary elements for maximum receptor expression. Ready-to-use solution: avoids cloning steps, DNA ready to transfect. Easy protocol and detection: the whole procedure is simple, with minimal handling. Neomycin resistance. Ampicillin bacterial selection.

Includes: Includes for 15 µg: ExpressMAX™ GPCR ORF Clones (Cat. No. -A & B) - 15 µg ExpressMAX™ Mammalian Expression Vector (1 mg/mL) Mammalian Transfection Kits (Cat. No. -PLUS): - 15 µg ExpressMAX™ Mammalian Expression Vector (1 mg/mL) - 0.2 mL CANFAST™ Transfection Reagent (15 µg = 15 assays)

Applications: Functional assays, as protein immobilization, cellular localization and other functional assays. High-throughput and large scale protein production and purification. Reverse transfection arrays and nucleic acid programmable protein arrays (NAPPA).