Canvax M-MLV Reverse Transcriptase P0074

M-MLV Reverse Transcriptase, encoded moloney murine leukemia virus, purified from e.coli (200 u/µl)

M-MLV Reverse Transcriptase (MMLV-RT), encoded by Moloney Murine Leukemia Virus (MMLV) is an RNAdependent DNA polymerase that synthesizes the cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. MMLV-RT will also extend primers hybridized to single-stranded DNA. Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase. Source: purified from E.coli strain harboring a plasmid that directs the synthesis of modified form of MMLV-RT. MW: 69 KDa.

Main Features:

Concentration: 200 U/µL. Full Solution: includes Reaction Buffer 5x. High Quality: free of DNAse, RNase or protease contamination. Purity: >90% as judged by SDS-polyacrylamide gels with blue staining. Final Product Size: 7 kb or less. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes: - M-MLV Reverse Transcriptase (200 U/µL) - Reaction Buffer 5x

Applications: RT PCR. Synthesis of cDNA. mRNA 5’-end Mapping by Primer Extension Analysis. End-labeling of DNA. Dideoxynucleotide Sequencing.