Canvax pSpark® II DNA Cloning Kit C0002-S

pSpark® II DNA Cloning Kit, for highly efficient, accurate & easy general cloning with classical mcs, without the use of toxic genes

pSpark® II is a highly efficient, accurate and easy-to-use DNA Cloning system based on a breakthrough technology for cloning blunt ended DNA generated by PCR with a proofreading or high fidelity DNA polymerases. The vector is prepared by digestion of pSpark® II at the EcoRV site before treating both ends to prevent vector self-ligation. The end treatment is supported by a proprietary know-how that guarantees a higher cloning efficiency than with simply a desphorylated vector.

Main Features:

Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions. Great efficiency: over hundreds positive colonies with few nanograms of insert. High stability: eliminates cloning bias or pitfalls. Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation. Powerful: clone from less than 1 ng/kb to up to 14 kb

Includes: Includes for 20 rxn: - 20 µL pSpark® II (20 ng/µL) - 20 µL T4 DNA Ligase (5U/Weiss) - 200 µL T4 DNA Ligase Buffer (5x) - 150 µL PEG 6000 (10x) - 5 µL Insert Control 1 kb (20 ng/µL)

Applications: General cloning. Clone PCR fragments included in a low amount. Cloning of PCR products amplified with High Fidelity polymerases. Cloning of PCR fragments generated with a blend of polymerases. Production of ssDNA. Blue/white screening for recombinants. In vitro transcription from T7/SP6 dual-opposed promoters.