Kf Streptococcal Agar 1034 Condalab

KF Streptococcal Agar is a selective medium for the isolation and enumeration of faecal enterococci in water, foodstuffs, and other materials. It is used for the plate count of enterococci in water samples and for determining the presence of Enterococcus faecalis in milk and its derivatives, as well as in other foods.

The isolation and enumeration of faecal enterococci is made according to APHA for the examination of water (1998) and foodstuffs (1992). Peptone mixture provides nitrogen, vitamins, minerals, and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group. Maltose and Lactose are the fermentable carbohydrates which are used as carbon and energy sources. Sodium glycerophosphate is a buffering agent and sodium azide is a selective agent that inhibits Gram-negative bacteria. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bacteriological agar is the solidifying agent.

The addition of TTC 1% Supplement (2,3,5-trypheniltetrazolium-chloride) allows faecal enterococci to develop a red colour as the result of the reduction of tetrazolium to formazan, an insoluble red pigment, by actively growing microbial cells.