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DMEM/HAM's F-12 without L-Glutamine Bioconcept 1-26F08-I

PART NO: BC-1-26F08-I

Price: €12.88

Out of Stock (Ships in approx. 1 week)

Dulbecco's MEM is the most widely used modification of BME. It contains four times the concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added and the bicarbonate concentration was increased. The standard formula is with 1000 mg/L glucose, the “high glucose” variation with 4500 mg/L. Dulbecco’s MEM was originally developed for the culture of mouse embryonic cells. Today it finds a broad application for serum free culture of normal and transformed mouse and chicken cells, e.g. 1:1 with Ham's F-12.

Specification

Inorganic Salts mg / L	mg / L
CaCl2 2H2O	154
CuSO4 5H2O	0.00125
Na2HPO4 anhydrous	71
Fe(NO3)3 9H2O	0.05
FeSO4 7H2O	0.417
MgCl2 6H2O	61
MgSO4 7H2O	100
KCI	311.8
NaCl	6999.5
NaH2PO4H2O	62.5
NaHCO3	2438
ZnSO4 7H2O	0.43
Other Components
1,4-Diaminobutane 2 HCI	0.08
D(+)-Glucose	3151
DL-alpha-Lipoic acid	0.105
Hypoxanthine	2.05
Linoleic acid	0.042
Phenol Red	8.1
Pyruvic acid sodium salt	55
Thymidine	0.365
Amino Acids
Glycine	18.75
L-Alanine	4.45
L-Arginine HCI	147.5
L-Asparagine H2O	7.5
L-Aspartic acid	6.65
L-Cysteine HCI H2O	17.56
Amino Acids mg / L
L-Cystine	24
L-Glutamic acid	7.35
L-Histidine HCI H2O	31.48
L-Isoleucine	54.45
L-Leucine	59.05
L-Lysine HCI	91.25
L-Methionine	17.24
L-Phenylalanine	35.48
L-Proline	17.25
L-Serine	26.25
L-Threonine	53.45
L-Tryptophan	9.02
L-Tyrosine	38.7
L-Valine	52.85
Vitamins
(+)-Biotin	0.00365
Choline chloride	8.98
D-Pantothenic acid calcium salt	2.24
Folic acid	2.65
myo-Inositol	12.6
Nicotinamide	2.02
Vitamin B1 HCI	2.17
Vitamin B2	0.219
Vitamin B6 HCI	2.031
Vitamin B12	0.68
To be added separately
L-Glutamine	365