ISF-1 is a serum-free, chemically defined medium for hybridoma cell culture and monoclonal antibody production. It contains Glutamine and does not require further supplementation. To protect cells from shear forces during production, surfactant is included in the medium. ISF-1 is not suitable for cholesterol dependent cell lines (e.g. NSO and its varients), without further supplementation of lipopprotein. The BSA used in ISF-1 is EDQM-certified. The addition of antibiotics should not be a substitute for proper sterile techniques. Therefore, use of antibiotics is in most cases neither necessary nor advised. However, in those instances where antibiotics are desired, ISF-1 has been shown to be compatible with the most used antibiotics (e.g. gentamycin, puromycin and amphotericin B).