Ham's F-10 without L-Glutamine 500 ml Bioconcept 1-13F01-I
PART NO: BC-1-13F01-I
Price: €13.72
Out of Stock (Ships in approx. 1 week)
Ham's F-10 Medium is a complex formula containing amino acids, vitamins and certain supplements and ingredients at optimum concentrations. It was originally developed for serum free culture of Chinese Hamster Ovary (CHO) cells, HeLa cell clones and mouse “L” cells. Ham's F-10 is today a widely established and popular medium for the growth of fastidious cell lines with serum or serum free, eg. diploid human cells, white blood cells for chromosomal analysis, primary explants of rat, chicken and rabbit.
Specification
| Inorganic Salts | mg / L |
| CaCl2 2H2O | 44 |
| CuSO4 5H2O | 0.0025 |
| Na2HPO4 anhydrous | 154 |
| FeSO4 7H2O | 0.834 |
| MgSO4 7H2O | 153 |
| KCI | 285 |
| KH2PO4 | 83 |
| NaCl | 7400 |
| NaHCO3 | 1200 |
| ZnSO4 7H2O | 0.0283 |
| Other Components | |
| D(+)-Glucose | 1100 |
| DL-alpha-Lipoic acid | 0.21 |
| Hypoxanthine | 4.1 |
| Phenol Red | 1.2 |
| Pyruvic acid sodium salt | 110 |
| Thymidine | 0.7 |
| Amino Acids | |
| Glycine | 7.5 |
| L-Alanine | 9 |
| L-Arginine HCI | 211 |
| L-Asparagine H2O | 15 |
| L-Aspartic acid | 13.3 |
| L-Cysteine | 25 |
| L-Glutamic acid | 14.7 |
| Amino Acids mg / L | |
| L-Histidine HCI H2O | 23 |
| L-Isoleucine | 2.6 |
| L-Leucine | 13.1 |
| L-Lysine HCI | 29 |
| L-Methionine | 4.48 |
| L-Phenylalanine | 4.96 |
| L-Proline | 11.5 |
| L-Serine | 10.5 |
| L-Threonine | 3.6 |
| Tryptophan | 0.6 |
| L-Tyrosine | 1.81 |
| L-Valine | 3.5 |
| Vitamins | |
| (+)-Biotin | 0.024 |
| Choline chloride | 0.7 |
| D-Pantothenic acid Calcium salt | 0.715 |
| Folic acid | 1.32 |
| myo-Inositol | 0.542 |
| Nicotinamide | 0.615 |
| Vitamin B1 HCI | 1 |
| Vitamin B2 0 | 0.375 |
| Vitamin B6 HCI | 0.205 |
| Vitamin B12 | 1.36 |
| To be added separately | |
| L-Glutamine | 146 |
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