HotBegan™ Hot Start Taq DNA Polymerase MasterMix, for a specific, highly efficient & reliable amplifications of dna up 160 fg in ready-to-use format

HotBegan™ Hot Start Taq DNA Polymerase MasterMix (2x) an optimized ready-to-use solution containing HotBegan™ Taq DNAPolymerase (hot start performance), dNTPs, MgCl2 and Stabilizers. It is inactive at Room Temperature and includes all material needed except Template and Primers. It is a Horse-Power™ Taq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until a denaturation step occurs. The heat-labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and non-specific products. Like Horse-Power™ Taq DNA Polymerase, the enzyme has 5’?3’ polymerase activity and a weak 5’?3’ exonuclease activity but no 3’?5’ exonuclease activity (proofreading). Before enzyme activation none of the enzyme activities are detectable.

Main Features:

Time-saving solution: ready-to-use MasterMix. High specificity: minimizes unspecific amplification. Efficient: prevents or minimizes primer-dimer and nonspecific products. Great sensitivity: amplifies from femptograms of DNA targets. Inactive: at Room Temperature. Optimized: adds extra nucleotides (preferentially adenine) without template at 3´ends leaving 3´overhangs PCR fragments. Powerful: amplification of targets up to 5 kb. Convenient: available in kits and MasterMix solution. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes: - 2 x 1.25 mL HotBegan™ Hot Start Taq DNA Polymerase MasterMix, which includes HotBegan™ Hot Start Taq DNA Polymerase (0.1 U/µL), 2x PCR Buffer, 0.4 mM of each dNTP, 4 mM Mg2+, 4% Glycerol. - 2 x 1.25 mL Nuclease-free water.

Applications: Real time PCR. RT-PCR and quantitative RT-PCR. Genotyping with Taqman Probes. PCR fragments amplification for TA or GC Cloning. Amplification from a limited DNA template or low copy number genes.