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Home > Life Science > Genomics > DNA and RNA isolation > Plasmid Purification-Kits I-Blue Mini / Midi > I-Blue MIDI Plasmid Kit 25 preps IBI Scientific IB47181

I-Blue MIDI Plasmid Kit 25 preps IBI Scientific IB47181
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I-Blue MIDI Plasmid Kit 25 preps IBI Scientific IB47181

PART NO: 4AJ-4661764
Price: €301.72
Qty In Stock: 10

The I-Blue Midi Plasmid Kit (25 preps) uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50 – 150 ml of cultured bacterial cells.

Included in the kit, there is an I-Blue Lysis Buffer (an option color indicator), which helps to prevent common handling errors, making sure that efficient cell lysis and neutralization is achieved.

- Sample Size: 50 – 150ml of cultured bacterial cells
- Operation: anion-exchange resin column, gravity flow
- Expectant Yield: up to 400µg of pure transfection grade plasmid DNA form 100ml cultured bacterial cells
- Elution Volume: 500µl – 2ml
- Operation Time: 80 minutes or less

Sample Packs Available: 4AJ-4661772

Specifications I-Blue Midi Plasmid Kit
Format: Anion-Exchange
Operation: Gravity Flow
Binding Capacity: 500 µg
Filter Column: Yes
Sample Type: Cultured bacterial cells (high-copy = 50-100 ml, low-copy = 100-150 ml)
Sample Concentration: 50-150 ml
Fragment Size: 1-20 kb
Typical Yield: 200-500 µg
Elution Volume: 500 µl- 2 ml
Operation TIme: <80 minutes


How It Works?



A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. I-Blue lysis buffer is added during the cell re-suspension step, then PM2 buffer is added.

After the addition of the PM2 buffer the sample will begin to turn blue during mixing, when the sample is completely blue ,complete lysis has been achieved. Colourless areas or brown clumps indicate incomplete lysis and mixing should continue until the sample is entirely blue. During the neutralization step, PM3 buffer is added and shaking the tube vigorously will begin to turn the sample back to a neutral color. Once the sample is completely colourless, neutralization is complete.

Due to the high efficiency of the Midi Column, the entire procedure can be completed in 80 minutes without ultracentrifuges, HPLC, or other toxic reagents. The purified plasmid DNA is suitable for transfection, sequencing reactions, ligation, PCR, in-vitro transcription, microinjection, restriction enzyme digestion, and gene gun.

FAQ


What is the formulation for the PW buffer in the I-Blue Midi plasmid kit?

The exact formulation of the IBI PW buffer is proprietary, but this is QIAGAN’s formulation which will work fine. 1.0 M NaCl, 50 mM MOPS, pH 7.0, 15% Isopropanol

Catalogue Number4.661 764
ManufacturerIBI Scientific
Manufacturer P/nIB47181
For25 preps
TypeI-Blue MIDI
PK1
DescriptionGravity flow
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