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How Microplate Sealing Films Handle Pressure

How Microplate Sealing Films Handle Pressure

Microplates: A Basic Overview

Microplates are widely utilized in research, academia, and industry for quantitative analysis and testing of analytes and biochemical processes. Biological, chemical, or physical processes occur within the wells of the microplate, where they may be seen and analysed. Although 96-well plates remain the industry standard, microplates with six to nine thousand wells are now available in a variety of shapes and materials to suit any purpose.    

What exactly are microplate sealing films?

Sealing films function as a barrier between the wells' substrates and the surrounding environment. They are intended to keep evaporation, contamination, cross-contamination, and leakage at bay during storage, transportation, and testing. Films must be robust and secure, as well as enable for colorimetric and other forms of analysis without interfering with the results. Different varieties of films are intended to be compatible with certain equipment and circumstances, such as temperature and pressure fluctuations. This specificity is achieved by the use of appropriate materials, dependable adhesives, and skilled production.

Can the film withstand the heat of PCR testing?

During the denaturation step of PCR (Polymerase Chain Reaction) testing, DNA strands are separated using heat. Temperature variations are repeated throughout this process. Microplates for thermocycling are often constructed of polypropylene or, at a lower cost, polycarbonate. While these materials have some thermostability, the expansion and contraction of the plates puts strain on the seal's integrity. It is also critical to select the appropriate grade and kind of film to guarantee that seals remain intact at each and every well throughout the operation.      

Pressure to perform – Adhesive

Microplates are manufactured from a variety of materials that are suitable for a variety of applications. Microplate sealing films and adhesives are in the same category. Film adhesives must endure stress caused by chemical changes in samples, temperature variations, and pressure changes of up to 2 bar in each well. Adhesive material must operate consistently and reliably, dealing with variations within wells as well as changes in microplate dimension and shape. This flexibility should not jeopardize performance; any adhesive should be strong enough to work properly.

Quality Control

Choosing a substandard or wrong film is inconvenient at best, and costly, time-consuming, and harmful at worst. Microplate seals must be suitable for purpose since they are subjected to such severe stress. If you are search for a product with a reputation for dependability and constant high quality. The Lab Unlimited stocks  top manufacturers  with rigorous product testing and have experience and a thorough grasp of the needs in each phase.


When it comes to microplate sealing films, sacrificing quality is nearly always a bad idea. After investing time, effort, and money, you must be certain that you have picked items and equipment on which you can rely. Choose a manufacturer you can rely on to safeguard and retain the reputation you've worked so hard to build.


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