The test kit was developed to obtain or concentrate DNA fragments from agarose gels, PCR or other enzymatic processes.
The agarose gel is dissolved, the enzymes are denatured and the DNA fragments will bind to the glass fibre matrix in the spin column. Wash buffers (containing ethanol) are used to remove contaminants and a low salt elution buffer is used to recover the purified DNA fragments. Recoveries are 90 - 95 % for PCR clean-up. With this kit, PCR purification and gel extraction procedures can be performed, so that a second test kit is not necessary.
|Sample size:||up to 300 mg of agarose gel / up to 100 µl of PCR product|
|Binding capacity:||10 µg DNA|
|Fragment size:||<10 kb|
|Typical yield:||80-90% gel extraction / 90-95% PCR clean-up|
|Operation Time:||<20 min.|