Real-time PCR-system QPCR-500

High specification, economically priced real-time thermocycler. The QPCR-500 system has a unique high thermal block uniformity (±0.1 °C). This uniformity as well as validated sample volumes of down to 5 µl enable an optimized reaction run time for 40 cycles down to only 15 minutes. The uniform temperature across the plate is achieved with a unique heating and cooling system that quickly enables a uniform temperature of ±0.1 °C in every well of the block within a fraction of a second of reaching each well temperature. This high uniformity eliminates the need for triplicates which reduces reagent running costs. QPCR-500 accommodates a unique and economical 48-well PCR plate, the size of which is only 1/8 the size of a standard 96-well plate. The well format mirrors a 384-well plate therefore allowing the use of a 16-channel micropipette. The small plate cuts reagent costs in half whilst still producing a strong fluorescent signal. Applications include e.g. absolute and relative quantification, Allelic Discrimination and High Resolution Melt.

Features:

• Validated volume per well: 5 to 20 µl
• High temperature uniformity of ±0.1 °C across the whole block instantly after every temperature change
• 40 cycles in 40 minutes before optimization, and down to only 15 minutes when optimized
• Genotype in 4 minutes with over 99 % accuracy
• Patented "Adaptive LED Control" minimizes optical artifacts and prevents premature detector saturation
• Dual LED excitation (452 nm to 486 nm and 542 nm to 582 nm)
• 4 emission filters (505 nm to 545 nm, 562 nm to 596 nm, 604 nm to 644 nm, 665 nm to 705 nm)
• Data is always collected in all four filters for all wells
• Distinguishes 5000 and 10000 template copies with 99 percent confidence

• 48-well block
• MIQE compliant
• 400 analyte specific qPCR reagent kits available
• No calibration required
• Calibrated dyes: SYBR^®, FAMTM, HEXTM, ROXTM, Cy^®5.
• Additional dyes within the filter wavelength range are supported with no additional calibration required
• Unlimited software licence
• 2 years warranty